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. 2010 Jul;78(1):69–80. doi: 10.1124/mol.110.063727

Fig. 10.

Fig. 10.

Effect of RanBP10 and RanBP9 on D1 receptor expression and signaling. A, RanBP10 or RanBP9 was coexpressed with the D1 receptor in HEK293T cells followed by assessment of DA-stimulated cAMP accumulation. The curves on the left are representative experiments, whereas the histograms on the right represent averaged data from three experiments using 100 μM DA as the stimulus. Expression of either RanBP10 or RanBP9 decreased maximal DA-stimulated cAMP accumulation compared with control (D1 receptor + empty vector): Emax ± S.E.M. values for D1R + RanBP10 and D1R + RanBP9 were 47 ± 6 and 46 ± 10% of control, respectively. B, saturation radioligand binding experiments were performed on membranes prepared from control cells (D1 receptor + empty vector) or cells expressing the D1 receptor along with either RanBP10 or RanBP9. The curves on the left are representative experiments, whereas the histograms on the right represent averaged Bmax data from three experiments. RanBP9 coexpression reduced the D1 receptor Bmax value to 60 ± 14% of control. (∗, p < 0.05, ∗∗, p < 0.005, paired Student's t test).