Fig. 11.

RanBP10 and RanBP9 increase basal D₁ receptor phosphorylation. In situ phosphorylation experiments were performed on HEK293T cells expressing the D₁ receptor, D₁ receptor + RanBP10, and D₁ receptor + RanBP9 as described under Materials and Methods. Cells were incubated with media (control) or DA (10 μM) for 10 min. Top, autoradiogram of D₁ receptor immunoprecipitates from a representative in situ phosphorylation assay. The lanes in the gel correspond to the bars at the bottom. Bottom, average values of band densities for each condition. The data are normalized as the percentage of control for each individual experiment. The histograms represent the mean ± S.E.M. from four independent experiments (∗, p < 0.05, ∗∗, p < 0.01, ∗∗∗, p < 0.001, analysis of variance followed by Bonferroni pair-wise comparisons).