Fig. 12.

The RanBP9 and RanBP10-induced increase of D₁ receptor phosphorylation is blocked by PKC inhibitors. HEK293T cells expressing the D₁ receptor, D₁ receptor + RanBP10, and D₁ receptor + RanBP9 were treated with the PKC inhibitors Gö6983 (10 μM) or Gö6976 (10 μM) for 60 min followed by the assessment of basal phosphorylation using an in situ phosphorylation assay as described in Fig. 11. Top, autoradiogram of D₁ receptor immunoprecipitates from a representative in situ phosphorylation assay. The lanes in the gel correspond to the bars at the bottom. Bottom, average values of band densities for each condition. The data are normalized as the percentage of control for each individual experiment. The histograms represent the mean ± S.E.M. from three independent experiments (analysis of variance followed by Bonferroni pair-wise comparisons; ∗, p < 0.05, ∗∗, p = 0.056).