Fig. 5.

Effect of RanBP9 overexpression on ethanol-dependent attenuation of PKC activities. Epitope-tagged PKCs were immunoprecipitated from solubilized membrane fractions prepared from HEK293T cells coexpressing either PKCγ (A and B) or PKCδ (C and D) with vector, RanBP10, or RanBP9. The kinase activities of the isozyme-specific immunoprecipitates were directly assessed using an in vitro kinase assay as described under Materials and Methods. For each condition, the kinase activities were measured under basal or lipid-activated (plus phosphatidylserine and diacylglycerol) conditions in the presence or absence of 100 mM ethanol. Data are presented as the mean ± S.E.M. of at least three independent experiments. Results that are significantly different from the control groups are indicated as ∗, p < 0.05; ∗∗∗, p < 0.001, paired Student's t test.