Fig. 6.

RanBP10 and RanBP9 coimmunoprecipitate with the D₁ receptor. The FLAG-D₁ receptor was expressed in HEK293T cells either alone or with RanBP10-GFP or RanBP9-GFP. Membranes were prepared and solubilized followed by immunoprecipitation of the D₁ receptor using anti-FLAG antisera as described under Materials and Methods. After resolution of the precipitates on SDS-PAGE, the gels were blotted with antisera directed to GFP (top) to detect RanB10 or RanBP9 or antisera directed to the D₁ receptor (bottom). The experiment shown is representative of three independent experiments.