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. 2010 Aug;136(2):189–202. doi: 10.1085/jgp.200910374

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© 2010 Yusifov et al.

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Figure 3. — Mol wt determination using MALLS. (A) The normalized absorption at 280 nm (continuous line) of eluted WT-RCK1 is superimposed with its mol wt distribution (red circles) calculated from light-scattering data. (B) A Debye plot with Zimm formalism is shown using the light-scattering data from 14 detectors for the sample at elution volume 2.893 ml. Extrapolation of the fit to zero angle reveals a mean mol wt of eluted protein corresponding to 331 kD. (C) Relative protein abundance (calculated from UV absorbance) of samples eluted between volumes 2.6 and 3.3 ml binned according to mol wt, calculated from MALLS analysis. According to this cumulative distribution, 76.3% of eluted protein had mol wt between 316 and 398 kD, with a mean of 352 ± 24 kD.