Table 1.

Primers and Probes Used for RT-PCR and Real-Time RT-PCR

Gene	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Probe Sequence
RT-PCR
TNFA	AGGTCCTCTTCAAGGGCCAAG	CTCTGGCAGGGGCTCTTGAT
IL1B	GACAAAATACCTGTGGCCTTGG	TTCTGCTTGAGAGGTGCTGATG
S100A9	GTCACTGCTGGAACGCAGCTTA	TTTGTGTCCAGGTCCTCCATGA
HPRT	gctcaagagctactgtaatgac	CATATCCAACAACAAACTTGTCTG
Real-time PCR	TaqMan forward primer (5′-3′)	TaqMan reverse primer (5′-3′)	TaqMan probe sequence (5′-3′)
HPRT	GCAGTATAATCCAAAGATGGTCAAGGT	TCTGGCCTATATCCAACACTTCGA	TTCACCAGCAAGCTTG
MUC19	TGACACCTGCTGTGAAATTGGA	GGAAGCACCAATCTCATAGTCAGT	CTGTGAACCAAGAACATG

Abbreviation: RT-PCR, reverse transcriptase–polymerase chain reaction.