Table 1. Genes involved in cell cycle and DNA damage response are upregulated in the forebrains of induced CK-p25 mice.
Microarray analysis was carried out as described in Experimental Procedures, and genes were organized into functional groups based on gene ontology. Genes with multiple gene ontology annotations (e.g. PCNA) were arbitrary placed into one of the groups. ‘Fold Δ’ indicates fold change in expression levels for p25 compared to controls at 2 weeks of induction. The entire list of genes with significantly altered expression (Table S1) and cell cycle/DNA damage related genes (Table S2) are shown in the Supplementary Data section.
| Function | Gene name | Accession | Fold Δ |
|---|---|---|---|
| Cell cycle related genes | Cyclin A2 | X75483 | 3.78 |
| Cyclin B1 | NM_007629 | 10.58 | |
| Cyclin E1 | NM_007633 | 1.94 | |
| Cyclin E2 | AF091432 | 4.9 | |
| Cdc28 | NM_016904 | 2.4 | |
| Cdc28 regulatory subunit 1 | NM_025415 | 5.11 | |
| Cdc2a (cdk1) | NM_007659 | 8.47 | |
| Cdc20 | BB041150 | 4.09 | |
| Cell division associated 1 (Nuf2R) | AK010351 | 2.16 | |
| Polo-like kinase 4 | AI385771 | 2.9 | |
| Geminin | NM_020567 | 2.27 | |
| Mcm2 | NM_008564 | 5.01 | |
| Mcm3 | C80350 | 6.27 | |
| Mcm4 | BC013094 | 3.06 | |
| Mcm6 | NM_008567 | 6.04 | |
| Mcm7 | NM_008568 | 3.78 | |
| DNA primase, p49 subunit | J04620 | 3.19 | |
| DNA primase, p58 subunit | NM_008922 | 1.67 | |
| p21/WAF | AK007630 | 2.55 | |
| Proliferating cell nuclear antigen (PCNA) | BC010343 | 2.47 | |
| Ki-67 proferation antigen | X82786 | 16.14 | |
| E2F-1 | NM_007891 | 5.04 | |
| Transcription factor DP-1 | BG075396 | 1.73 | |
| DNA damage responsive genes | Rad51 | NM_011234 | 31.77 |
| Rad51 associated protein | BC003738 | 9.93 | |
| Topoisomerase II alpha | BM211413 | 6.02 | |
| DNA methyltransferase (cytosine-5) 1 | NM_010066 | 1.67 | |
| Flap endonuclease | BB393998 | 2.65 | |
| MutS homolog 6 | U42190 | 1.66 | |
| Ligase I | NM_010715 | 3.99 | |
| DNA polymerase epsilon | NM_011132 | 37.8 | |
| DNA polymerase delta 1, catalytic subunit | BB385244 | 1.93 | |
| Pmaip1 | NM_021451 | 5.23 | |
| Deoxyuridine triphosphatase | AF091101 | 1.65 | |
| Ribonucleotide reductase M2 | BB758819 | 5.65 | |
| Replication protein A1 | BB491281 | 1.64 | |
| Replication protein A2 | AK011530 | 2.13 | |
| Uracil DNA glycosylase | BC004037 | 3.24 | |
| Chromatin assembly factor 1b | NM_011132 | 5.81 | |
| BRCA1 | U31625 | 5.45 | |
| Checkpoint 1 | C85740 | 8.06 | |
| Mad2-like 1 | NM_019499 | 2.59 |