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. Author manuscript; available in PMC: 2010 Jul 29.
Published in final edited form as: J Biol Chem. 2007 Feb 27;282(17):12475–12483. doi: 10.1074/jbc.M609280200

FIGURE 3. Detecting mRNA for hyaluronan synthase genes in keratocytes.

FIGURE 3

Total RNA was extracted from primary bovine keratocytes cultured in serum-free medium or treated for 6 h with FBS and TGFβ as in Fig. 1. RNA was reverse-transcribed using random primers, and DNA was amplified for 35 cycles using primers representing unique regions from bovine HAS1, 128 bp; HAS2, 134 bp; and HAS3, 234 bp (Table 1). The products were separated on 6% acrylamide gels and stained using SYBR Gold. Lane 7 shows DNA molecular size standards.