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Primary bovine keratocytes were exposed to FBS and TGFβ as in Fig. 1, for the intervals shown, and the total RNA was analyzed by qRT-PCR for each gene product compared with 18 S RNA in the same sample as described under “Experimental Procedures.” Bars show the standard error of triplicate analyses carried on duplicate cultures. For each gene, mRNA abundance at time 0 was set to 1.
