TABLE 1.
Primers and siRNA
| Gene | Primer | Sequence |
|---|---|---|
| HAS1 | Forward | AGGCCTGGTACAACCAGAAG |
| Reverse | TCTCCGAGTAGCAGCGAGAC | |
| Taqman probe |
FAM-AGCATGGGCTATGCCACCAAGTACAC-TAMRA | |
| siRNA 1 | GUGCUGCTCTCACUCUAUA | |
| siRNA 2 | GGAACAACCUCCUGCAGCA | |
| siRNA 3 | GCGAGUGGCUGUACAAUGC | |
| siRNA 4 | ACGCGUGGAUGACCUACGA | |
| HAS2 | Forward | CCGTCATCACTGGGTTCTTC |
| Reverse | TAAGGCAGCTGGCAAAAGAT | |
| Taqman probe |
FAM-TCATTGCCACGGTAATCCAGCTCTTC-TAMRA | |
| siRNA | CCUUGGAAUCACAGCUGCUUAUAUU | |
| HAS3 | Forward | CCAACCGAGTCCTGAGTCTT |
| Reverse | TGTATGACCGTGGCAATGAG | |
| Taqman probe |
FAM-CTCCCGGAAGTAAGACTTGCTCCAGC-TAMRA |