Skip to main content
. Author manuscript; available in PMC: 2010 Oct 2.
Published in final edited form as: J Mol Biol. 2010 Feb 6;397(3):799–813. doi: 10.1016/j.jmb.2010.02.001

Table 1.

Kinetic parameters for RNA compaction under splicing conditions. D1 and D1356 RNAs (1 nM) were studied at 8 mM MgCl2 and 30 °C in the absence and in the presence of Mss 116 (WT or SAT mutant, 20 nM), as indicated.

RNA Conditions kfast, min-1 Ampl. fast, %* kslow*102, min-1 Ampl. slow, %*
D1 No protein 0.23±0.06 27±1 0.56±0.06 32±9
Mss 116WT, ATP 1.3±0.1 73±7 2.9±0.3 17±5
Mss 116WT, no ATP 1.1±0.3 21±3 1.5±0.2 56±4
Mss 116SAT, ATP 1.4±0.2 21±4 2.0±0.4 57±3
Mss 116SAT, no ATP 0.71±0.04 26±1 0.85±0.07 60±10
D1356 No protein 0.16±0.03 46±4 0.6±0.2 20±6
Mss 116WT, ATP 1.4±0.2 78±3 3.0±0.5 15±5
Mss 116WT, no ATP 0.35±0.02 43±4 1.5±0.4 41±1
Mss 116SAT, ATP 1.6±0.3 36±7 4±1 45±7
*

These values do not include the amplitude of an initial collapse observed at a "0" time point, which constitutes ~10%.