Table 2.
Kinetic parameters of the D1 RNA compaction at low magnesium ion concentrations. D1 (1 nM) was studied at 1 and 3 mM MgCl2 and 30 °C in the absence and in the presence of WT Mss 116 (20 nM) and ATP (1 mM). Values for 8 mM MgCl2 are from Table 1.
| Mss 116 | [MgCl2], mM | kfast, min-1 | Ampl fast, %* | kslow* 102,min-1 | Ampl. slow, %* |
|---|---|---|---|---|---|
| No protein | 1 | >5 | 28 ± 4 | 0.8 ± 0.2 | 10 ± 1 |
| 3 | 3 ± 1 | 26 ± 1 | 1.1 ± 0.5 | 27 ± 5 | |
| 8 | 0.23 ± 0.06 | 27 ± 1 | 0.56 ± 0.06 | 32 ± 9 | |
| Mss 116WT, ATP | 1 | >5 | 40 ± 3 | 0.7 ± 0.1 | 15 ± 7 |
| 3 | 3.1 ± 0.9 | 37 ± 7 | 1.9 ± 0.6 | 42 ± 3 | |
| 8 | 1.3 ± 0.1 | 73 ± 7 | 2.9 ± 0.3 | 17 ± 5 |
*
These values do not include the amplitude of an initial collapse observed at a "0" time point, which constitutes ~10%.