Table 3.
Kinetic parameters for SE RNA compaction and lariat formation under splicing conditions. SE RNA (1 nM) was studied at 8 mM MgCl2 and 30 °C in the absence and in the presence of Mss 116 (WT or SAT mutant, 20 nM), as indicated.
| Conditions | kfast, min-1 | Ampl. fast, %* | kslow*102, min-1 | Ampl. slow, %* | klar*102, min-1 |
|---|---|---|---|---|---|
| No protein | 0.1±0.05 | 8±1 | 0.075±0.003 | 80±3 | 0.155±0.007 |
| Mss 116WT, ATP | 0.8±0.1 | 54±1 | 0.9±0.2 | 28±1 | 0.3±0.08 |
| Mss 116WT, no ATP | 0.05±0.01 | 26±2 | 0.5±0.1 | 62±3 | 0.25±0.06 |
| Mss 116SAT, ATP | 0.06±0.01 | 63±3 | 0.55±0.06 | 18±3 | 0.33±0.08 |