Table 3.
MUC2 Expression Levels by Run of RT-PCRa
| Run | Sample | MUC2 Expression | Fold Increase |
|---|---|---|---|
| Run 1 | CI-01 | 3.59 | |
| CI-02 | 0.35 | ||
| CI-03 | 0.80 | ||
| RecOM-1 | 11.40 | 7.22 | |
| RecOM-2 | 15.05 | 9.52 | |
| RecOM-3 | 1.16 | 0.73 | |
| OME-1 | 51.20 | 32.41 | |
| OME-2 | 36.62 | 23.18 | |
| OME-3 | 13.72 | 8.68 | |
| OME-4 | 7.35 | 4.65 | |
| Run 2 | CI-04 | 0.20 | |
| CI-05 | 1.03 | ||
| CI-06 | 4.85 | ||
| RecOM-4 | 1.36 | 0.67 | |
| OME-5 | 3.76 | 1.85 | |
| OME-6 | 2.72 | 1.34 | |
| OME-7 | 3.51 | 1.73 | |
| Run 3 | CI-05 | 3.59 | |
| CI-06 | 0.35 | ||
| RecOM-5 | 11.58 | 5.88 | |
| RecOM-6 | 2.96 | 1.50 | |
| RecOM-7 | 2.83 | 1.44 | |
| RecOM-8 | 30.57 | 15.52 | |
| RecOM-9 | 13.94 | 7.08 | |
| RecOM-10 | 2.64 | 1.34 | |
| Run 4 | CI-14 | 0.27 | |
| CI-15 | 2.24 | ||
| RecOM-11 | 17.54 | 13.98 | |
| RecOM-12 | 1.89 | 1.50 | |
| OME-8 | 1.50 | 1.19 | |
| OME-9 | 2.86 | 2.27 | |
| Run 5 | CI-05 | 0.48 | |
| CI-06 | 6.79 | ||
| CI-14 | 2.87 | ||
| CI-15 | 1.08 | ||
| CI-19 | 1.17 | ||
| RecOM-13 | 12.97 | 5.23 | |
| RecOM-14 | 10.29 | 4.15 | |
| RecOM-15 | 2.00 | 0.81 | |
| RecOM-16 | 18.34 | 7.40 | |
| RecOM-17 | 2.96 | 1.19 | |
| OME-10 | 6.26 | 2.53 |
Abbreviations: CI, cochlear implant controls; OME, otitis media with effusion; RecOM, recurrent otitis media; RT-PCR, reverse transcription–polymerase chain reaction.
a
MUC2 expression levels by run of real-time RT-PCR. MUC2 values represent the comparison of threshold cycle of MUC2 to the housekeeping gene HPRT for each sample.7 “Fold increase” indicates the fold increase of each sample over the mean of CI samples for that run of real-time RT-PCR.