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. 2010 Jul 19;120(8):2699–2714. doi: 10.1172/JCI39506

Figure 6. HIF-2α is required for TPA- and TG-induced inflammation.

Figure 6

(A–G) TPA-induced acute ear inflammation is impaired in myeloid HIF-2α–deficient mice. (A–D) Ear skin was painted with either acetone (control) or TPA; ear tissue was harvested 24 hours later and stained with H&E. Scale bars: 20 μm. (E and F) Ear tissue was stained with Gr-1 antibody to confirm that the majority of infiltrating cells are PMNs. Scale bars: 20 μm. (G) Infiltrating cells (based on H&E staining) and Gr-1+ cells were counted under high-power (×40) fields (n = 3, *P < 0.05). (H) Myeloid HIF-2α–deficient mice displayed reduced total number of TG-elicited peritoneal exudate macro­phages (PEMs) (n = 5, ***P < 0.001). Arrowheads in C, D, E, and F indicate neutrophils.