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. 2010 Jul 1;120(8):2795–2804. doi: 10.1172/JCI39679

Figure 1. Zebrafish ccm3 proteins are highly conserved with the human ortholog CM3, and ccm3 is necessary during early zebrafish development.

Figure 1

(A) Alignment of the predicted amino acid sequences of zebrafish ccm3a and ccm3b with that of human CCM3. Identical residues are shaded in gray. Vertical lines indicate exon boundaries. The amino acids encoded by CCM3 exon 5 (hExon5) and ccm3a/b exon 3 (zExon3) are indicated. (B) Expression pattern of ccm3a and ccm3b in zebrafish embryos. ccm3a and ccm3b are ubiquitously expressed at the 60% epiboly and 12-second stages. At 24 hpf, ccm3a and ccm3b are expressed most strongly in the head. (C–G) Loss of ccm3a+ccm3b or ccm3b alone results in early embryonic lethality in zebrafish. Shown are 24-hpf zebrafish embryos following injection of ccm3aX2 mismatch control morpholino (C, 5 ng/embryo); a morpholino (ccm3a/bATG) designed to block translation of both ccm3a and ccm3b (D, 0.5 ng/embryo); or morpholinos specifically interfering the splicing of ccm3a or ccm3b: ccm3bX4 morpholino (E, 4 ng/embryo), ccm3aX2 morpholino (F, 3 ng/embryo), and ccm3aX4 morpholino (G, 4 ng/embryo). C, F, and G are composites of 2–3 images taken of the same embryos.