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. 2010 Jul 1;120(8):2795–2804. doi: 10.1172/JCI39679

Figure 6. STK deficiency, like CCM deficiency, results in actin stress fiber formation and elevated Rho-A activity in human endothelial cells.

Figure 6

(A) Phalloidin staining of actin stress fibers in HUVECs treated with control siRNA and siRNAs targeting CCM2, CCM3, STK24, STK25, or STK24+STK25 is shown. (B) The percentage of cells with central actin stress fibers following the siRNA treatments described in A is shown. Shown are mean and SEM. n = 6; *P < 0.05, ***P < 0.0001 by Student’s t test. (C) Upregulation of Rho-A activity is associated with deficiency of CCM proteins or STKs. Rho-A–GTP levels were measured in endothelial cells treated with the indicated siRNAs (top bands) and compared with total cellular Rho-A (bottom bands). (D) Fold change in Rho-A activation following treatment of HUVECs with siRNAs targeting the indicated genes or with LPA (10 μM, 30 minutes). Shown are mean and SEM. n = 3. *P < 0.05, **P < 0.01 by Student’s t test. Scale bars: 20 μm.