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. 2010 Jul 12;120(8):2900–2909. doi: 10.1172/JCI41069

Figure 1. Generation of the GnRH-IRKO mouse.

Figure 1

(A) The IR gene with loxP sites (large arrows) flanking exon 4 before (top panel) and after (bottom panel) recombination by Cre. Primers used for genotyping and detection of the loxP flanked or truncated IR gene are labeled p1, p2, and p3. (B) PCR of DNA was performed using primers indicated in A. The loxP flanked allele is indicated by the 320-bp band, while the excised IR gene is indicated by the 220-bp band. Hrt, heart; Liv, liver; Ut, uterus; Ov, ovary; Cer, cerebellum; Pit, pituitary; Hyp, hypothalamus; Blk, blank.