Figure 1. Sirtuin gene expression in mouse eggs and preimplantation embryos.

(A) Conventional RT-PCR analysis. Eggs and preimplantation embryos were collected for RNA sampling from the oviducts or uteri at the appropriate time for each stage as follows: egg, 1-cell, 2-cell, approximately 4- to 8-cell, morula (M), and blastocyst (BL). Ppia expression served as an internal control. (B) Relative quantification of sirtuin mRNA levels by real-time RT-PCR.