Figure 5. DT-mediated basophil ablation in Mcpt8^DTR mice abolishes acquired tick resistance.

Mcpt8^DTR mice were infested once or twice with ticks as in Figure 1. DT or vehicle (PBS) was administered twice, 2 days before and 2 days after the initiation of the second infestation. The relative tick repletion for each group is shown in A. Data are shown as mean ± SEM, n = 3 each. (B and C) Numbers of basophils and mast cells in the tick-feeding sites 4 days after the initiation of the second infestation. Data are shown as mean ± SEM, n = 3 each. Data shown in A–C are representative of at least 3 experiments. **P < 0.01; *P < 0.05.