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. Author manuscript; available in PMC: 2010 Jul 29.
Published in final edited form as: Curr Cancer Drug Targets. 2009 Sep;9(6):777–788. doi: 10.2174/156800909789271503

Fig. 4.

Fig. 4

Expression profiles of factors involved in tumor angiogenesis during metronomic chemotherapy. Relative levels of rat VEGF, TSP-I, PLGF, HIF-1α, and mouse VEGF receptors 1 and 2 in 9L tumors were quantified by real-time PCR (qPCR) using SYBR Green 1 chemistry following the second of two weekly CPA treatments (140mg/kg i.p.). Total RNA was isolated from frozen tumor samples (0.1–0.4 g) using TRIzol reagent according to the manufacturer’s protocol. Ct values determined for each mRNA were normalized to the 18S rRNA content of each RNA sample and are plotted as fold increase over untreated tumor samples. Data are represented as mean ± SE relative mRNA levels of at least four individual tumors per group. *: p< 0.05 and **: p< 0.01.