Figure 2. Repeated antigen stimulation impacts memory CD8+ T cell phenotype, function and tissue distribution.
A) Phenotype of OT-I T cells in the spleen 90 days post infection. Numbers inside dot plots represent the percentage of cells positive for the indicated molecules. B) Peptide-stimulated intracellular cytokine staining (ICS) of OT-I T cells from spleen. Numbers represent the percentage of OT-I T cells that stain positive for a given cytokine combination. C) OT-I T cell frequency in total CD8+ T cells in tissues was normalized to the frequency in PBL. Data are mean +/− SD for 3 mice per group.