Table 2. Comparison of gene expression between 1° and 4° memory CD8⁺ T cells.

Gene expression in 1° and 4° memory OT-I T cells. FDR q-value <0.01 defined statistical significance. Genes with the most pronounced A) increase or B) decrease in mRNA quantity in 4° compared to 1° memory CD8 T cells. Genes are ranked according to fold changes in mRNA quantity. Molecules in bold letters are discussed in the text.

A)	Transcriptional upregulation quaternary vs. primary memory
rank	Gene Symbol	Alias	change
1	Klra10	LY49J	9.51
2	Havcr2	TIM-3	9.15
3	Klre1	NKG2I	9.08
4	Klra14	LY49N	7.79
5	Klra9	LY49I	7.33
6	Klri2		7.13
7	AA467197		5.96
8	Gzmb		5.42
9	Klrg1	KLRG-1	5.38
10	Capg		5.22
11	ENSMUSG00000073493		5.13
12	Gstm5		5.12
13	Car5b		5.03
14	Cd244	2B4	4.63
15	6330403K07Rik		4.52
16	Lamc1		4.49
17	EG433024		4.31
18	Ccl9		4.19
19	I830127L07Rik		4.09
20	Serpinb1a		4.05
21	Ms4a4a		3.91
22	Fcgr2b		3.72
23	Esm1		3.59
24	4933431E20Rik		3.58
25	Zeb2		3.42
26	Cx3cr1		3.37
27	Anxa1		3.30
28	Bhlhe40		3.29
29	Tspan2		3.22
30	Crybg3		3.22
31	Lonrf3		3.18
32	A930038C07Rik		3.18
33	Nebl		3.10
34	Itgam	CD11b	3.09
35	Arhgef12		3.09
36	Klra8	LY49H	3.08
37	Tef		3.06
38	Mmp25		3.06
39	Emp1		3.02
40	Srxn1		3.02
41	Akr1e1		2.98
42	Chn2		2.97
43	Ern1		2.96
44	Rora		2.92
45	Fasl		2.83
46	Tmem97		2.80
47	Pcdh21		2.78
48	Vmn2r86		2.78
49	5730494M16Rik		2.74
50	Pik3ap1		2.74
51	Klrc3	NKG2E	2.73
53	Slamf7	CRACC	2.69
63	Cd80		2.44
70	Slamf1	SLAM	2.31
76	Ccr5		2.23
79	Il2ra	CD25	2.19
83	Il18rap		2.16
86	Klrc2	NKG2C	2.13
97	Il12rb2		2.07

B)	Transcriptional downregulation quaternary vs. primary memory
rank	Gene Symbol	Alias	change
1	Sell	CD62L	−15.35
2	Myc		−15.11
3	Tlr1		−11.18
4	Wfikkn2		−10.18
5	Tnfsf8	CD30L	−9.36
6	D17H6S56E-5		−7.10
7	Insr		−6.54
8	Xcl1		−6.29
9	F2rl1		−6.08
10	Snhg1		−5.84
11	Cd55		−5.53
12	Mboat1		−5.44
13	Dapl1		−5.13
14	Sidt1		−4.49
15	Slamf6	NTB-A	−4.49
16	Rpp38		−4.47
17	Gm885		−4.40
18	Treml2		−4.16
19	Cxcr3		−4.12
20	Rnf144a		−4.11
21	Pdk1		−4.04
22	Aff3		−4.03
23	Tnfrsf26	Tnfrh3	−3.94
24	Rgs10		−3.90
25	Nsg2		−3.87
26	Fam169b		−3.84
27	Art2b		−3.62
28	Atp6v0d2		−3.58
29	Hook1		−3.54
30	Itgax	CD11c	−3.46
31	Ms4a4c		−3.46
32	Insr		−3.43
33	Larp1		−3.37
34	Ly6e	Sca-2	−3.32
35	Spin2		−3.31
36	A530040E14Rik		−3.28
37	2900016B01Rik		−3.26
38	Actn1		−3.26
39	Ccr7		−3.20
40	Qpct		−3.19
41	Qtrtd1		−3.10
42	Capn11		−3.10
43	Dpp4		−3.08
44	Cdh1		−3.05
45	BC059842		−3.03
46	A530040E14Rik		−3.02
47	A530040E14Rik		−3.02
48	Ssbp2		−2.98
49	A530040E14Rik		−2.91
50	A530040E14Rik		−2.91
62	Ltb	lymph. B	−2.66
76	Il2		−2.44
82	Tcf7	Tcf-1	−2.36
113	Satb1		−2.12