Table 2. Designs for bacterial metagenome experiments.

S ()		Eq. 3 Reads	Eq. 4 Reads	Eq. 5 Reads	(5,50,95)% minimax
100	4	313476	336963	365122	3.33, 3.49, 3.65
200	4	642683	766366	807394	3.27, 3.44, 3.59
400	4	1315088	1764672	1806689	3.21, 3.38, 3.51
100	5	489834	535031	584071	4.32, 4.51, 4.75
200	5	1000506	1217621	1290028	4.17, 4.43, 4.61
400	5	2040273	2800352	2877594	4.13, 4.37, 4.59
100	6	669257	739677	811931	5.26, 5.63, 5.92
200	6	1363646	1683594	1791344	5.20, 5.57, 5.83
400	6	2774570	3868329	3986651	5.06, 5.36, 5.59

Table 2 provides the numbers of reads of size determined to give 95% probability of assembling contigs of at least size in bacterial ( = 2000000,  = Uniform(1000000,3000000)) metagenomics problems as a function of the number of species or in the pool. Calculations and relationships between both experimental terms and the required number of reads and planned and observed contig sizes are as described in Table 1.