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Effect on the L0045 expression in EHEC by the presence or absence of grlA. (A) L0045 expressed from pQE60_L45 in the bacterial lysates when different EHEC mutant strains harbored the specified plasmids. (B) Comparison of L0045 detected in the EHEC ΔgrlA strain (left) and JM109 (right) with or without GrlA expressed from pACYC184_GrlA. Note: pQE60_L45 and pACYC184_GrlA are compatible when co-transformed into the same host bacteria.
