Figure 2. Western blots confirm the radioligand binding method for ranking expression.

Monoclonal antibody to a non-polymorphic region of the β₂AR protein was utilized to confirm the radioligand binding method for ranking expression phenotypes of the β₂AR haplotypes. Shown is a single experiment revealing higher expression of haplotypes IV-1 and IV-3 compared to IV-4 and VI-2 β₂AR protein at the expected molecular weight (∼79 kDa). These results are consistent with those from radioligand binding of Figure 1. The control antibody was to Na⁺/K⁺-ATPase, a cell membrane protein.