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. 2010 Jul 29;5(7):e11822. doi: 10.1371/journal.pone.0011822

Figure 5. Cell-free and cell-based estrogenic assays.

Figure 5

(A) Dose-response curves for competitive binding assay. Calycosin and 17-β-estradiol (E2) at the concentrations shown competitively bind with (i) ERα and (ii) ERβ, which caused displacement of Fluormone™ ES2 from ER; (B) Dose-response curves for GeneBLAzer β-lactamase reporter-gene assay. (i) Agonistic activities and (ii) antagonistic activities of calycosin at ERα and ERβ were determined in ERα-UAS-bla GripTite™ and ERβ-UAS-bla GripTite™ cell lines respectively. Results are presented as mean±SEM. (n≥2 independent experiments), P<0.01 between different ER subtypes followed by two-way ANOVA.