Figure 6. The effects of calycosin, raloxifene and tamoxifen in SIVs of Tg(fli1:EGFP).

(A) Controls: were treated with 0.1% DMSO at 96 hpf, showing no effect on vessel formation (B–E) were treated with 10 µM raloxifene, 3 µM tamoxifen, 10 µM 17-β-Estradiol and 100 µM calycosin at 72 hpf for 24 h. (a–e) Enlarged SIV region (×4.5) of A–E respectively. Abnormal phenotype of blood vessel formation in SIVs was indicated by white arrow, showing slight increase in vessel diameter. Significant increase in vessel diameter was indicated by thick white arrow.