Figure 1. Summary results for the primary gain-of-function screen for activators of HSF1.

A total of 13724 full-length genes were co-transfected with a human HSP70B promoter driving firefly luciferase and a constitutively expressed Renilla luciferase control. (A) Relative activity of the 13724 full-length genes normalized to the negative plasmid control on each plate (pCMV-Sport6 Empty). Each data point represents the average of four independent replicates. The line labeled 6*STDV_Control represents a cut-off of 6 standard deviations above the mean of the negative control. (B) The quantile distribution of the 13724 data points from the primary screen compared to a normal distribution (straight line).