Table 2. Validation of potential HSF1 regulators using RNAi.

	Firefly Luciferase Promoter			Directional FDR Corrected
	(log2Ratio vs. SCRAM_1)a			p-valueb
siRNA Duplex	HSP70B	TK	CMV	HSP70B vs.TK	HSP70B vs.CMV
PSMD3_1	−1.06±0.20	−1.23±0.53	−0.99±0.61	0.35	−0.4
PSMD3_2	−1.36±0.27	−1.53±0.53	−1.38±0.49	0.35	0.43
ZCCHC12_1	−2.25±0.31	−2.06±0.37	−1.96±0.42	−0.32	−0.25
ZCCHC12_2	−1.59±0.27	−1.43±0.10	−1.37±0.21	−0.24	−0.2
HMX2_1	−1.33±0.31	−1.00±0.32	−0.84±0.53	−0.18	−0.17
HMX2_2	−1.56±0.24	−1.12±0.53	−1.01±0.45	−0.18	−0.099
JMJD2C_1	−0.48±0.23	−0.89±0.34	−0.99±0.24	0.12	0.05
JMJD2C_2	−1.31±0.10	−2.17±0.86	−2.16±0.76	0.12	0.09
PRKCI_1	−0.18±0.05	0.26±0.04	0.42±0.20	−0.00016	−0.0064
PRKCI_2	−0.77±0.15	−0.12±0.35	−0.21±0.30	−0.038	−0.039
KCTD10_1	−0.52±0.23	−0.28±0.23	−0.29±0.13	−0.18	−0.14
KCTD10_2	0.39±0.29	−0.05±0.12	0.18±0.22	0.064	0.24
KIAA1826_1	−0.05±0.22	−0.35±0.25	−0.22±0.35	0.14	0.31
KIAA1826_2	−0.07±0.21	−0.13±0.17	0.02±0.29	0.36	−0.36
GTSE1_1	−0.47±0.17	−0.83±0.53	−0.82±0.55	0.21	0.22
GTSE1_2	0.01±0.14	−0.13±0.12	−0.11±0.24	0.17	0.3
SCRAM_2 (neg.)	−0.05±0.18	−0.26±0.17	−0.14±0.23	0.15	0.35
HSF1_1 (pos.)	−2.40±0.22	0.01±0.19	0.11±0.29	−0.00012	−0.00021
HSF1_2 (pos.)	−3.37±0.25	−0.13±0.32	−0.04±0.24	−0.00012	−0.000078

^aMean ± SD for 4 biological replicates.

^bStatistical significance between the HSP70B reporter and the TK and CMV reporters was calculated using a two sample t-test. The p-values were corrected for multiple comparisons using false discovery rate. Negative p-values denote a reduction in activity versus the control reporter. SCRAM_1 and SCRAM_2 denote negative RNAi controls. HSF1_1 and HSF1_2 denote positive RNAi controls for the reduction of HSF1 activity.