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. 2010 Jul 16;5:9. doi: 10.1186/1750-2187-5-9

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Copyright ©2010 Mishra et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of knockdown of β-catenin and Snail expression on G17-induced migration. (A). Subconfluent AGSE cells were transiently transfected with 100 nM each of either control-siRNA, or β-catenin-siRNA, or Snail-siRNA or a combination of β-catenin and Snail siRNA. They were wounded 48 hours post-transfection and treated with G17 and pictures were obtained as described under 4A. (B) The distance of migration of the wounded edges were measured as in 4B and represented as "Average Gap". (C) AGSE cells transfected in A and treated with G17 were analyzed by Western Blot analysis utilizing the indicated antibodies.