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. 2010 Aug 1;21(15):2568–2577. doi: 10.1091/mbc.E10-01-0049

Figure 8.

Figure 8.

Effects of A-CREB on the up-regulation of CGRP mRNA expression by protons. (A) F11-Trpv1 cells were transfected with CGRP(350)-Luc constructs together with empty vector or the A-CREB expression vector as indicated. Cells were treated with pH 5.5 medium for 24 h. The data are shown as fold activation normalized to control (mean ± SD). *p < 0.05 versus pH 5.5. (B) F11-Trpv1 cells were infected with adenovirus carrying A-CREB and cultured for 2 d. Cell lysates were used for immunoblotting with anti-FLAG and anti-β-actin antibodies. (C) F11-Trpv1 cells infected with or without adenovirus carrying A-CREB were treated with pH 5.5 medium for 8 h. The expression of CGRP mRNA was determined with real-time PCR. The data are shown as fold activation normalized to control (mean ± SD). *p < 0.05 versus pH 5.5. (D) DRGs isolated from rats were infected with adenovirus carrying A-CREB and cultured for 2 d. DRG lysates were analyzed using immunoblotting with anti-FLAG and anti-β-actin antibodies. (E) DRGs infected with or without adenovirus carrying A-CREB were treated with pH 5.5 medium for 12 h. The expression of CGRP mRNA was determined with real-time PCR. The data are shown as fold activation normalized to control (mean ± SD). *p < 0.05 versus pH 5.5.