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. 2010 Aug 1;21(15):2598–2610. doi: 10.1091/mbc.E09-07-0609

Figure 2.

Figure 2.

S100A4−/− BMMs exhibit reduced migration toward CSF-1. BMMs were CSF-1 starved for 16 h before seeding in an 8-μm transwell. The CSF-1–stimulated migration of each cell population is expressed as the fold change relative to unstimulated cells. Data represent the mean ± SEM for three independent experiments performed in triplicate. (A) Chemokinesis (3 h) of wild-type and S100A4−/− BMMs; 20 ng/ml CSF-1 was present in both the upper and lower chambers. (B) Chemotaxis (3 h) of wild-type and S100A4−/− BMMs; 20 ng/ml CSF-1 was present in only the lower chamber. (C) Chemotaxis (5 h) of wild-type BMMs, S100A4−/− BMMs, and S100A4−/− BMMs expressing either TurboRFP or human S100A4; 20 ng/ml CSF-1 was present in only the lower chamber. Inset, expression of S100A4 in primary BMMs. β-Actin was used as a loading control. 1, S100A4−/− BMMs transduced with a TurboRFP lentivirus; 2, S100A4−/− BMMs transduced with the human S100A4 lentivirus; 3, S100A4−/− BMMs; and 4, wild-type BMMs.