Table 1.
Yeast strains
| Strain | Designation | Genotype | Reference |
|---|---|---|---|
| YPH499 | WT | MAT a ade2-101 his3-200 leu2-1 lys2-801 trp-63 ura3-52 | Sikorski and Hieter (1989) |
| NYY0-1 | ARF1 | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 | Yahara et al. (2001) |
| NYY11-1 | arf1-11 | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 | Yahara et al. (2001) |
| NYY17-1 | arf1-17 | MAT a ade2::arf1-17::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 | Yahara et al. (2001) |
| NYY18-1 | arf1-18 | MAT a ade2::arf1-18::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 | Yahara et al. (2001) |
| YAS1031A | ARF1 Dcp2-GFP | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1032A | arf1-11 Dcp2-GFP | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1033A | arf1-17 Dcp2-GFP | MAT a ade2::arf1-17::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1034A | arf1-18 Dcp2-GFP | MAT a ade2::arf1-18::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS2428 | ARF1 Edc3-eqFP611 eIFG2-GFP | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 EDC3::EDC3-eqFP611-kanMX4 eIFG2::eIFG2-yEGFP-TRP1 (K. lactis) | This study |
| YAS2429 | arf1-11 Edc3-eqFP611 eIFG2-GFP | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 EDC3::EDC3-eqFP611-kanMX4 eIFG2::eIFG2-yEGFP-TRP1 (K. lactis) | This study |
| YAS1681 | sar1-D32G Dcp2-GFP | MAT a ade2-101 his3-200 leu2-1 lys2-801 trp-63 ura3-52 SAR1::LEU2 (K. lactis) pMYY3-1(Ycp[sar1-D32G TRP1]) DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1133 | sec21-1 Dcp2-GFP | MAT α ade2-101 his3-200 leu2-1 lys2-801 trp-63 ura3-52 sec21-1 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1134 | sec27-1 Dcp2-GFP | MAT a ade2-101 his3-200 leu2-1 lys2-801 trp-63 ura3-52 sec27-1 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1131 | Δgea2 gea1-19 Dcp2-GFP | MAT a ura3-52 leu2,3-112 his3-200 GEA2::HIS3 gea1-19 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1135 | sec6-4 Dcp2-GFP | MAT a ade2-101 his3-200 leu2-1 lys2-801 trp-63 ura3-52 sec6-4 CHS6::CHS6-9myc-TRP1 (K. lactis) DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1877 | sec3-2 Dcp2-GFP | MAT a ura3 leu2 trp1 sec3-2 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1880 | sec4-8 Dcp2-GFP | MAT a ura3 leu2 lys sec4-8 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS1878 | sec2-41 Dcp2-GFP | MAT a ura3 leu2 his3 lys2 trp1 ade2 sec2-41 DCP2::DCP2-yEGFP-kanMX4 | This study |
| YAS2235 | ARF1 Pub1-GFP | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 PUB1::PUB1-yEGFP-kanMX4 | This study |
| YAS1945 | ARF1 Dcp2-GFP Δhog1 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 HOG::LEU2 (K . lactis) | This study |
| YAS1946 | arf1-11 Dcp2-GFP Δhog1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 HOG::LEU2 (K. lactis) | This study |
| YAS1685 | ARF1 Dcp2-GFP Δsho1 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SHO1::LEU2 (K. lactis) | This study |
| YAS1686 | arf1-11 Dcp2-GFP Δsho1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SHO1::LEU2 (K. lactis) | This study |
| YAS1947 | ARF1 Dcp2-GFP Δsko1 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SKO1::LEU2 (K. lactis) | This study |
| YAS1948 | arf1-11 Dcp2-GFP Δsko1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SKO1::LEU2 (K. lactis) | This study |
| YAS1967 | ARF1 Dcp2-GFP Δslt2 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SLT2::LEU2 (K. lactis) | This study |
| YAS1968 | arf1-11 Dcp2-GFP Δslt2 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SLT2::LEU2 (K. lactis) | This study |
| YAS1986 | ARF1 Dcp2-GFP Δhog1 Δslt2 | MAT a ade2::ARF11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 HOG1::LEU2 (K. lactis) SLT2::URA3 (K. lactis) | This study |
| YAS1987 | arf1-11 Dcp2-GFP Δhog1 Δslt2 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 HOG1::LEU2 (K. lactis) SLT2::URA3 (K. lactis) | This study |
| YAS2092 | ARF1 Dcp2-GFP Δhog1 Δslt2 Δcnb1 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 HOG1::loxP SLT2::loxP CNB1::LEU2 (K. lactis) | This study |
| YAS2292 | arf1-11 Dcp2-GFP Δhog1 Δslt2 Δcnb1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 HOG1::loxP SLT2::loxP CNB1::LEU2 (K. lactis) | This study |
| YAS2575 | ARF1 Dcp2-GFP Δypk1 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 YPK1::LEU2 (K. lactis) | This study |
| YAS2010 | arf1-11 Dcp2-GFP Δypk1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 YPK1::LEU2 (K. lactis) | This study |
| YAS2021 | ARF1 Dcp2-GFP Δcnb1 | MAT a ade2::ARF11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 CNB1::LEU2 (K. lactis) | This study |
| YAS2051 | arf1-11 Dcp2-GFP Δcnb1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 CNB1::LEU2 (K. lactis) | This study |
| YAS2088 | ARF1 Dcp2-GFP Δcmk1 Δcmk2 | MAT a ade2::ARF11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 CMK1::LEU2 (K. lactis) CMK2::URA3 (K. lactis) | This study |
| YAS2089 | arf1-11 Dcp2-GFP Δcmk1 Δcmk2 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 CMK1::LEU2 (K. lactis) CMK2::URA3 (K. lactis) | This study |
| YAS2090 | ARF1 Dcp2-GFP Δcmk1 Δcmk2 Δcnb1 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 CMK1::loxP CMK2::loxP CNB1::LEU2 (K. lactis) | This study |
| YAS2091 | arf1-11 Dcp2-GFP Δcmk1 Δcmk2 Δcnb1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 CMK1::loxP CMK2::loxP CNB1::LEU2 (K. lactis) | This study |
| YAS2586 | arf1-11 Dcp2-GFP Δgcn2 | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 GCN2::URA3 (K. lactic) | This study |
| YAS2588 | arf1-11 Dcp2-GFP Δire1 | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 IRE1::URA3 (K. lactic) | This study |
| YAS2296 | ARF1 cmd1-3 Dcp2-GFP | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 CMD1::cmd1-3 | This study |
| YAS2580 | arf1-11 cmd1-3 Dcp2-GFP | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 | This study |
| pHS47(cmd1-3 URA3) CMD1::kanMX4 | |||
| YAS2294 | ARF1 Dcp2-GFP Δpat1 | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 PAT1::LEU2 (K. lactis) | This study |
| YAS2295 | arf1-11 Dcp2-GFP Δpat1 | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 PAT1::LEU2 (K. lactis) | This study |
| YAS2297 | sec6-4 Dcp2-GFP Δpat1 | MAT a ade2-101 his3-200 leu2-1 lys2-801 trp-63 ura3-52 sec6-4 CHS6::CHS6-9myc-TRP1 (K. lactis) DCP2::DCP2-yEGFP-kanMX4 PAT1::LEU2 (K. lactis) | This study |
| YAS1097 | ARF1 Dcp2-GFP Δscd6 | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SCD6::LEU2 (K. lactis) | This study |
| YAS1098 | arf1-11 Dcp2-GFP Δscd6 | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-yEGFP-kanMX4 SCD6::LEU2 (K. lactis) | This study |
| YAS2500 | sec6-4 Dcp2-GFP Δscd6 | MAT a ade2-101 his3-200 leu2-1 lys2-801 trp-63 ura3-52 sec6-4 CHS6::CHS6-9myc-TRP1 (K. lactis) DCP2::DCP2-yEGFP-kanMX4 SCD6::LEU2 (K. lactis) | This study |
| YAS1294 | ARF1 Dcp2-9myc | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-9myc-TRP1 (K. lactis) | This study |
| YAS1295 | arf1-11 Dcp2-9myc | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DCP2::DCP2-9myc-TRP1 (K. lactis) | This study |
| YAS1693 | ARF1 Dhh1-9myc | MAT a ade2::ARF1::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DHH1::DHH1-9myc-TRP1 (K. lactis) | This study |
| YAS1694 | arf1-11 Dhh1-9myc | MAT a ade2::arf1-11::ADE2 ARF1::HIS3 ARF2::HIS3 ura3 lys2 trp1 his3 leu2 DHH1::DHH1-9myc-TRP1 (K. lactis) | This study |
| YAS2576 | arf1-11 Pat1-GFP | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 PAT1::PAT1-yEGFP-TRP1 (K. lactis) | This study |
| YAS2578 | arf1-11 Scd6-GFP | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 SCD6::SCD6-yEGFP-kanMX4 | This study |
| YAS1153 | ARF1 Dhh1-GFP | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DHH1::DHH1-yEGFP-kanMX4 | This study |
| YAS1154 | arf1-11 Dhh1-GFP | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 DHH1::DHH1-yEGFP-kanMX4 | This study |
| YAS2153 | ARF1 Cmd1-GFP | MAT a ade2::ARF1::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 CMD1::CMD1-yEGFP-kanMX4 | This study |
| YAS2154 | arf1-11 Cmd1-GFP | MAT a ade2::arf1-11::ADE2 arf1::HIS3 arf2::HIS3 ura3 lys2 trp1 his3 leu2 CMD1::CMD1-yEGFP-kanMX4 | This study |