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Strong activation of CFTR by UTP in well-differentiated primary cultures of HBE cells. (A) Isc after additions of amiloride (10 μM), forskolin (10 μM), CFTRinh-172 (10 μM), UTP (100 μM), and CaCCinh-A01 (30 μM), a CaCC-selective inhibitor. (B) Cells were pretreated with CFTRinh-172 (10 μM) to block CFTR Cl− current (gray line). Forskolin and UTP-induced CFTR-dependent Isc (inhibited by CFTRinh-172, 10 μM) is summarized in bar graph format (error bars, SE; n = 7).
