Table 1.

Binding of CLIP-170 mutants to MT tips and lattice

Mutated site(s)	Binding to MTs in Taxol-treated cellsa		Length of MT tips, live imaging, μm
	Live imaging	Staining
1. Wild type	−	−	1.26 ± 0.28
in the third serine-rich domain
2. Ser309-311A	++	++	1.8 ± 0.4; p < 0.0001b
Ser309-311E	−	−	1.0 ± 0.19; p < 0.0001
3. Ser313A	++	++	1.36 ± 0.26; p < 0.001
Ser313 E	+	+	1.2 ± 0.20
4. Ser314A	−	−	1.2 ± 0.26
5. Ser316-317A	N.A.c	−	1.22 ± 0.3
6. Ser319-320A	++	++	1.4 ± 0.36; p < 0.0001
Ser319E	N.A.	−	1.0 ± 0.24; p < 0.0001
7. Ser323-324A	−	−	1.4 ± 0.43; p < 0.001
8. Ser326-327A	−	−	1.2 ± 0.26
9. Ser330A	−	−	1.2 ± 0.2
10. Ser339-340A	−	−	1.22 ± 0.3
11. Ser347A	+	+	1.21 ± 0.24
Ser347E	+	+	1.10 ± 0.12
12. Ser309-311-326-327A	++	N.A.	1.84 ± 0.5; p < 0.0001
13. Ser309-347A	+	N.A.	N.A.
14. Ser309-311-320A	++	N.A.	1.85 ± 0.46; p < 0.0001
15. Ser309-311-313-319-320A	++	++	3.04 ± 0.6; p < 0.0001
16. Ser309-311-313-319-320 E	−	−	0.7 ± 0.15; p < 0.0001
in the second serine-rich region
17. S146A	+	N.A.	1.34 ± 0.28
18. S149-192A	−	N.A.	1.14 ± 0.2
19. T188A	−	N.A.	1.0 ± 0.19; p < 0.0001
20. S194A	+	N.A.	1.2 ± 0.18
21. S203A	+	N.A.	1.2 ± 0.24

^a ++, strong binding; +, moderate binding; −, no binding.

^b p values were obtained based on t test compared with the length of nonmutated CLIP-170.

^c N.A., not applicable.