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Coexpression and tandem purification of recombinant MBP-IFT70/CrDYF-1 and SIIT-IFT46. Shown here is the Coomassie Blue stained gel of samples from each step of the tandem purification; the first two lanes following the markers contain the insoluble and soluble fractions of bacterial cell lysates. Note that both proteins copurify after tandem purification with amylose and StrepTactin affinity resin. F/T stands for the flow-through proteins that did not bind to the resistive resins.
