Figure 5.

IFT70/CrDYF-1 is partially associated with the axoneme. (A) Procedure for the preparation of the flagellar fractions. This procedure is essentially the same as described in Huang et al. (2007). (B) Stoichiometrically equivalent levels of whole flagella, matrix, membrane plus axoneme, membrane proteins, and bare axoneme (labeled on the top) were isolated according to the procedure described in A. Note that, like other IFT particle proteins, a portion of IFT70/CrDYF-1 remains associated with the axoneme. The samples were separated by 10% SDS-PAGE and analyzed by Western blotting.