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. 2010 Aug 1;21(15):2696–2706. doi: 10.1091/mbc.E10-03-0191

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© 2010 by The American Society for Cell Biology

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Figure 7. — IFT70/CrDYF-1 is required for flagellar assembly. (A) Levels of cellular IFT particle complex A proteins are elevated while Complex B proteins are reduced when IFT70/CrDYF-1 is reduced. The upper panels show the Western-blotting (WB) results of a few IFT particle proteins of whole cell extracts isolated from the knockdown strains miRNA-1 and miRNA-4, and the control cw92 cells. The IFT70/CrDYF-1 protein is indicated by an arrow on the left. The α-IFT70/CrDYF-1 antibody also recognized a non-specific band indicated by an asterisk (*) on the right. The lower panel is part of a Coomassie Blue-staining gel (Gel) to show the equal loading of all the samples. (B) IFT70/CrDYF-1 reduced miRNA cells assemble short flagella. The plot shows flagellar length distribution of cw92 (n = 116), miRNA-1 (n = 116), and miRNA-4 (n = 108) cells. The mean lengths of the flagella are listed.