Figure 10.

Suppressors of dhc-1(or195ts) lethality have distinct effects on cytoskeletal organization. Anti-α-tubulin antibody, phalloidin-rhodamine, and Hoechst staining of gonads (pachytene region) extruded from wild-type (N2) animals grown at 25°C on bacteria expressing the indicated dsRNA. Each of the RNAi knockdowns, cap-1, -2, dli-1, and lis-1 affects F-actin (red) organization and the morphology of the rachis, including nuclei (blue) positioning. cap-1 and -2 RNAi treatments caused an apparent increase in F-actin content and the formation of distinct short actin filaments (arrowheads). Microtubule (green) structures are largely unaffected by the treatments. Distal (D) and proximal (P) orientations of the gonad is indicated on the merged image. Scale bar, 10 μm.