Figure 11.

cap-1 and -2 knockdowns restore DHC-1 localization in dhc-1(or195ts) embryos. One-cell stage embryos from dhc-1(or195ts) animals (dhc-1) at the nonpermissive temperature (25°C) and fed with either control or cap-1 or -2 dsRNA-producing bacteria were stained with anti-β-tubulin (green), anti-DHC-1 (red), and Hoechst (blue). Individual channels are shown in grayscale, and the merged images are shown in color. Scale bar, 5 μm. To better visualize localization of dynein with respect to microtubules, the last column (5×) shows magnification of the white square–marked area of merged image; scale bar, 1 μm. cap-1 and -2 knockdown restored dynein localization from centrosomal location to near wild-type localization along microtubules.