Figure 3.

RNAi of lis-1 suppresses lethality of dhc-1(or195ts) animals. (A) Average number of viable hatched larvae laid by dhc-1(or195ts) animals in two consecutive 24-h periods on control bacteria or bacteria producing lis-1 dsRNA [lis-1(RNAi)]. Embryos were incubated an additional 24 h to assess hatching. n = 3; error bars, ±SEM. (B) Phalloidin-rhodamine and Hoechst staining of extruded gonads (pachytene region) to visualize F-actin (red) and DNA (blue), respectively, from dhc-1(or195ts) animals grown on control or lis-1 dsRNA-expressing bacteria at the nonpermissive temperature (25°C). 3D reconstruction of F-actin serial optical images. Depletion of lis-1 by RNAi greatly improved F-actin structure, comparable to N2 control (Figure 1A). Distal (D) and proximal (P) orientations of the gonad is indicated on the merged image. Scale bar, 10 μm.