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Coexpression of RUFY1 and either Rab14 or Rab4 induces expansion of endosomes. (A) HeLa cells were transfected with a plasmid encoding HA-RUFY1, EGFP-Rab14, EGFP-Rab4, or EGFP-Rab5. In (a), the cells were stained with antibody to HA. (B) HeLa cells were cotransfected with plasmids encoding HA-RUFY1 and either EGFP-Rab14 (a), EGFP-Rab4 (b), or EGFP-Rab5 (c), and stained with anti-HA antibody. Bars, 20 μm.
