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Coexpression of RUFY1(Δ507-517) and either Rab14 or Rab4 does not induce expansion of early endosomes. (A) HeLa cells transfected with a plasmid encoding EGFP-RUFY1 or EGFP-RUFY1(Δ507-517) were stained with anti-EEA1 antibody. (B and C) HeLa cells were cotransfected with a combination of plasmids encoding either EGFP-RUFY1 or EGFP-RUFY1(Δ507-517) and either HA-Rab14 (B) or HA-Rab4 (C), and stained with anti-HA antibody. Bar, 20 μm.
