Figure 1.

Schematic organization, idealized activation and surface expression of gp130/WSX-1, gp130/LIFR, gp130/OSMR wild type and gp130/WSX-1, gp130/LIFR, gp130/OSMR, and OSMR/GPL. (A) Scheme of the gp130 and WSX-1 receptors with the extracellular domains comprising immunglobulin-like domain (Ig, light green), cytokine binding domain (CBD, dark green), three fibronectin-like domains (FNIII, green), transmembrane domain (TM) and cytoplasmic domain (CD, black line). Dimerization is induced after binding of IL-27. All receptors were truncated 15 amino acids above the transmembrane domain and replaced by IL-15 or the sushi domain of IL-15Rα. (B) Cell surface expression of gp130, WSX-1, LIFR, OSMR, and GPL chimeric receptor in various stably transduced Ba/F3-gp130 cell lines analyzed by flow cytometry. The cell lines were labeled as described in Materials and Methods. Untransduced Ba/F3-gp130 cells were used as negative controls (unfilled histograms).