Figure 5. Locations of in vivo induced promoters on E. faecalis chromosome.

Promoters that were shown to be induced during persistence in Galleria mellonella larvae are located in open boxes and arrows below the boxes indicate the orientation in which promoters were cloned upstream from the recombinase gene in plasmid pCre2. Thick arrows represent Open Reading Frames located in the region neighboring these promoters. A: Pivi2 is located within a 1.2 kb-long region overlapping ef_0040, ef_0041 and the untranslated region between them. Pivi2 probably regulates ef_0041 expression. B: Pivi3 is located within a 1.1 kb-long fragment overlapping ef_3282 and ef_3283 and should control ef_3282 expression. C: Pivi4 is included in a 550 pb-long region overlapping ef_3198 and a small part of untranslated region between ef_3197 and ef_3198. This suggests that Pivi4 regulates the expression of the two-component system encoding operon ef_3196/7. D: Pivi5 is located in a 1.2 kb-long fragment overlapping ef_0091, ef_0092 and the untranslated region between them. Pivi5 could regulate ef_0092 and/or ef_0093 expression. E: Pivi6 is included in a 620 pb-long region overlapping ef_0376 and the untranslated fragment between this gene and ef_0377. So Pivi6 could control expression of ef_0377 and ef_0378 which seem to be co-transcribed.