Figure 1. Transgenic expression of Tat induces RelB synthesis in mouse brain.

6-week old Tat-transgenic mice were induced with doxycycline for the indicated periods of time. Total RNA was extracted from brain tissue, reverse transcribed using oligo-dT primers, and subjected to Real-Time SYBR Green RT-PCR amplification. Fold induction of Tat, RelB and RelA mRNA species was normalized to GAPDH and presented as a function of the expression level in D0 samples. Data represent mean ± SEM of four replicates for D0 and D14 samples, and five replicates for D3 samples. Statistical significance (***, p<0.001 or *, p<0.05) is denoted as compared to D0 samples.