Figure 3. RelB inhibits Tat-induced TNFα production in BV-2 microglia.

BV-2 cells (5×10⁶) were transiently transfected with either empty vector (pcDNA3.1-Myc/His-B), cRel, or RelB (10 µg), using Nucleofector (Amaxa/Lonza), and treated with Tat (100nM) for the indicated periods of time. TNFα release was measured by ELISA and normalized to total cellular protein content in the culture wells. Results are shown as mean ± SEM of values derived from two replicates from one representative experiment; two total experiments were performed. Statistical significance (p<0.05) is indicated, as compared to empty vector transfected cells (*).