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. 2010 Jul 29;6(7):e1001001. doi: 10.1371/journal.ppat.1001001

Figure 4. The MAVS-IKKβ pathway is important for γHV68 mRNA production.

Figure 4

(A and B) MAVS+/+ and MAVS−/− MEFs were infected with γHV68 (MOI = 0.01) and cells were harvested at two hours post-infection. Total DNA was extracted and viral genomes were analyzed by PCR and agarose gel electrophoresis (A) or quantitative real-time PCR (qRT-PCR) (B). (C and D) MEFs were infected with γHV68 as in (A). Total RNA was extracted and levels of γHV68 mRNA transcripts were examined by reverse transcription and PCR (C) or qRT-PCR (D) using primers specific for viral genes as indicated. I.E., immediate early; E, early. (E and F) 293T cells were transfected with the γHV68 BAC and a plasmid containing TRAF6. At 28 h post-transfection, levels of the γHV68 genome were determined by qRT-PCR (E) and levels of various γHV68 gene transcripts as indicated were determined by reverse transcription and qRT-PCR analyses (F). Data represent the mean ± SEM.